PURIFICATION OF MALIC ENZYME FROM BOVINE HEART-MITOCHONDRIA BY AFFINITY-CHROMATOGRAPHY

被引：8

作者：

CALDES, T ^{[1
]}

FATANIA, HR ^{[1
]}

DALZIEL, K ^{[1
]}

机构：

[1] UNIV OXFORD, DEPT BIOCHEM, OXFORD OX1 3QU, ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 100卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90233-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple two-step method for the purification of malic enzyme from bovine heart mitochondria in high yield is described. It consists of successive affinity chromatography steps on immobilized C8-(aminohexyl)-NADP and N6-(aminohexyl)-ADP. The molecular weight estimated by gel filtration of the homogeneous enzyme is 250,000 and the subunit molecular weight by SDS-polyacrylamide gel electrophoresis is 59,000. © 1979.

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页码：299 / 303

页数：5

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