LASER FLUORESCENCE DETECTOR FOR CAPILLARY ELECTROPHORESIS

被引：77

作者：

YEUNG, ES

WANG, PG

LI, WN

GIESE, RW

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,US DOE,AMES LAB,AMES,IA 50011

[2] NORTHEASTERN UNIV,COLL PHARM & ALLIED HLTH PROFESS,DEPT PHARMACEUT SCI,BOSTON,MA 02115

[3] NORTHEASTERN UNIV,BARNETT INST CHEM ANAL & MAT SCI,BOSTON,MA 02115

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 608卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)87107-J

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, rugged, and relatively inexpensive laser-based fluorometer for detection in capillary electrophoresis is described. This is assembled from commercially available components and requires minimal experience for operation. Yet, the detection performance is comparable to those achieved in laser laboratories with sophisticated layouts. As an example, detection of a 3 pM solution of fluorescein is demonstrated. The design principles of laser fluorometric detection are critically examined, and the special adaptation for the present detection arrangement is discussed.

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页码：73 / 77

页数：5

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