VASOACTIVE-INTESTINAL-PEPTIDE IN HUMAN NASAL-MUCOSA

被引：112
作者：
BARANIUK, JN
LUNDGREN, JD
OKAYAMA, M
MULLOL, J
MERIDA, M
SHELHAMER, JH
KALINER, MA
机构：
[1] NIH, CTR CLIN, INTENS CARE UNIT, BETHESDA, MD 20892 USA
[2] GEORGETOWN UNIV HOSP, DEPT OTOLARYNGOL, WASHINGTON, DC 20007 USA
[3] GEORGETOWN UNIV HOSP, DEPT PEDIAT, WASHINGTON, DC 20007 USA
来源：
JOURNAL OF CLINICAL INVESTIGATION | 1990年 / 86卷 / 03期
关键词：
Lactoferrin; Mucus; Parasympathetic nervous system; Respiratory glycoconjugates;
D O I：
10.1172/JCI114780
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Vasoactive intestinal peptide (VIP), which is present with acetylcholine in parasympathetic nerve fibers, may have important regulatory functions in mucous membranes. The potential roles for VIP in human nasal mucosa were studied using an integrated approach. The VIP content of human nasal mucosa was determined to be 2.84±0.47 pmol/g wet weight (n = 8) by RIA. VIP-immunoreactive nerve fibers were found to be most concentrated in submucosal glands adjacent to serous and mucous cells. 125I-VIP binding sites were located on submucosal glands, epithelial cells, and arterioles. In short-term explant culture, VIP stimulated lactoferrin release from serous cells but did not stimulate [3H]glucosamine-labeled respiratory glycoconjugate secretion. Methacholine was more potent than VIP, and methacholine stimulated both lactoferrin and respiratory glycoconjugate release. The addition of VIP plus methacholine to explants resulted in additive increases in lactoferrin release. Based upon the autoradiographic distribution of 125I-VIP binding sites and the effects on explants, VIP derived from parasympathetic nerve fibers may function in the regulation of serous cell secretion in human nasal mucosa. VIP may also participate in the regulation of vasomotor tone.
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收藏
页码：825 / 831
页数：7
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