A MITOCHONDRIAL-DNA DELETION IN NORMALLY AGING AND IN ALZHEIMER BRAIN-TISSUE

被引：47

作者：

BLANCHARD, BJ ^{[1
]}

PARK, T ^{[1
]}

FRIPP, WJ ^{[1
]}

LERMAN, LS ^{[1
]}

INGRAM, VM ^{[1
]}

机构：

[1] MIT,DEPT BIOL,77 MASSACHUSETTS AVE,CAMBRIDGE,MA 02139

来源：

NEUROREPORT | 1993年 / 4卷 / 06期

关键词：

ALZHEIMERS DISEASE; BRAIN AGING; DNA DELETION; MITOCHONDRIAL DNA; PCR; POLYMERASE CHAIN REACTION;

D O I：

10.1097/00001756-199306000-00051

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

By quantitative polymerase chain reaction (PCR) of total cellular DNA, the known 4977 bp deletion in human mitochondrial DNA (mtDNA(DELTA4977)) was not detected in rapidly dividing tissue such as placenta and lymphocytes, nor in brain tissue from fetuses and in frontal cortex from two individuals 24 and 56 years old. However, in frontal cortex from individuals 71-95 years 0.13% deleted/undeleted mtDNA was found, with no significant difference between Alzheimer patients (0.14%) and age-matched controls (0.12%). We hypothesize that the age-related accumulation of this deletion (and other expected deletions) contributes to the down-regulation of adenosine triphosphate (ATP) production in neurons and other non-dividing cells, a fundamental mechanism common to aging and Alzheimer's disease.

引用

页码：799 / 802

页数：4

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