CHEMICAL MODIFICATION OF THE STREPTOKINASE FUNCTIONAL-GROUPS IN THE SYSTEM CONTAINING SOLUBLE CARBODIIMIDE NUCLEOPHILES

被引：0
|
作者：
KAZYUCHITS, OA
NIKANDROV, VN
机构：
来源：
VOPROSY MEDITSINSKOI KHIMII | 1991年 / 37卷 / 05期
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D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Specific activity of streptokinase was decreased after incubation in the system containing I-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) - ethylene diamine. Under these conditions about 8 free amino groups and 38 free carboxylic groups were modified in the streptokinase molecule. The protein conformation and the state of tryptophane residues were altered. The streptokinase modified by means of EDC-ethylene diamine treatment lost its ability to form a stable complex with human plasminogen.
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页码：24 / 28
页数：5
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