PHYSIOLOGY AND MOLECULAR-GENETICS OF POLY(BETA-HYDROXY-ALKANOIC ACID) SYNTHESIS IN ALCALIGENES-EUTROPHUS

被引：228

作者：

STEINBUCHEL, A ^{[1
]}

SCHLEGEL, HG ^{[1
]}

机构：

[1] INST MICROBIOL, GRISEBACHSTR 8, W-3400 GOTTINGEN, GERMANY

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 03期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb00725.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Alcaligenes eutrophus genes for beta-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase and poly(beta-hydroxybutyric acid) synthase (PHB synthase) which comprise the three-step PHB-biosynthetic pathway, were cloned. Molecular studies revealed that these genes are organized in a single operon. The A. eutrophus PHB-biosynthetic genes are readily expressed in other bacteria, and DNA fragments harbouring the operon can be used as a cartridge to confer to other bacteria the ability to synthesize PHB from acetyl-CoA. The biochemical and physiological capabilities of A. eutrophus for the synthesis of a wide variety of polyhydroxyalkanoates are discussed.

引用

页码：535 / 542

页数：8

共 50 条

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