IL-6DBP, A NUCLEAR-PROTEIN INVOLVED IN INTERLEUKIN-6 SIGNAL TRANSDUCTION, DEFINES A NEW FAMILY OF LEUCINE ZIPPER PROTEINS RELATED TO C/EBP

被引：563
作者：
POLI, V [1 ]
MANCINI, FP [1 ]
CORTESE, R [1 ]
机构：
[1] EUROPEAN MOLEC BIOL LAB,W-6900 HEIDELBERG,GERMANY
来源：
CELL | 1990年 / 63卷 / 03期
关键词：
D O I：
10.1016/0092-8674(90)90459-R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We analyzed a family of proteins from hepatoma cell nuclei that bind to interleukin-6 responsive elements (IL-6REs) of several acute-phase genes. This family is characterized by leucine zipper domains compatible with that of the CCAAT/enhancer binding protein ( C EBP). A cDNA clone coding for a member of the family, IL-6DBP, was isolated; it is strongly homologous to C EBP in the region of the basic domain and in the leucine zipper sequence. IL-6DBP and C EBP can interact in vitro to form heterodimers that bind to DNA with the same specificity as the respective homodimers, and they can interact functionally in vivo. Both the DNA binding activity and the trans-activating capacity of IL-6DBP are induced in hepatoma cells by treatment with IL-6 through a posttranslational mechanism, implicating it as a nuclear target of IL-6 and as a mediator of the IL-6-dependent transcriptional activation of liver genes during the acute-phase response. © 1990.
引用
收藏
页码：643 / 653
页数：11
相关论文
共 51 条
[1]   A NUCLEAR FACTOR FOR IL-6 EXPRESSION (NF-IL6) IS A MEMBER OF A C/EBP FAMILY [J].
AKIRA, S ;
ISSHIKI, H ;
SUGITA, T ;
TANABE, O ;
KINOSHITA, S ;
NISHIO, Y ;
NAKAJIMA, T ;
HIRANO, T ;
KISHIMOTO, T .
EMBO JOURNAL, 1990, 9 (06) :1897-1906
[2]   REGULATION OF SYNTHESIS AND SECRETION OF MAJOR RAT ACUTE-PHASE PROTEINS BY RECOMBINANT HUMAN INTERLEUKIN-6 (BSF-2/IL-6) IN HEPATOCYTE PRIMARY CULTURES [J].
ANDUS, T ;
GEIGER, T ;
HIRANO, T ;
KISHIMOTO, T ;
TRANTHI, TA ;
DECKER, K ;
HEINRICH, PC .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 173 (02) :287-293
[3]   IDENTIFICATION OF SEQUENCES RESPONSIBLE FOR ACUTE-PHASE INDUCTION OF HUMAN C-REACTIVE PROTEIN [J].
ARCONE, R ;
GUALANDI, G ;
CILIBERTO, G .
NUCLEIC ACIDS RESEARCH, 1988, 16 (08) :3195-3207
[4]   TISSUE-SPECIFIC EXPRESSION, DEVELOPMENTAL REGULATION, AND GENETIC-MAPPING OF THE GENE ENCODING CCAAT ENHANCER BINDING-PROTEIN [J].
BIRKENMEIER, EH ;
GWYNN, B ;
HOWARD, S ;
JERRY, J ;
GORDON, JI ;
LANDSCHULZ, WH ;
MCKNIGHT, SL .
GENES & DEVELOPMENT, 1989, 3 (08) :1146-1156
[5]   DIMERS, LEUCINE ZIPPERS AND DNA-BINDING DOMAINS [J].
BUSCH, SJ ;
SASSONECORSI, P .
TRENDS IN GENETICS, 1990, 6 (02) :36-40
[6]   INDUCIBLE AND TISSUE-SPECIFIC EXPRESSION OF HUMAN C-REACTIVE PROTEIN IN TRANSGENIC MICE [J].
CILIBERTO, G ;
ARCONE, R ;
WAGNER, EF ;
RUTHER, U .
EMBO JOURNAL, 1987, 6 (13) :4017-4022
[7]  
Ciliberto G., 1989, ACUTE PHASE PROTEINS, P29, DOI [10.1007/978-1-4471-1739-1_3, DOI 10.1007/978-1-4471-1739-1_3]
[8]  
DARLINGTON GJ, 1989, ANN NY ACAD SCI, V557, P310
[9]  
de Simone V, 1988, Oxf Surv Eukaryot Genes, V5, P51
[10]   FIREFLY LUCIFERASE GENE - STRUCTURE AND EXPRESSION IN MAMMALIAN-CELLS [J].
DEWET, JR ;
WOOD, KV ;
DELUCA, M ;
HELINSKI, DR ;
SUBRAMANI, S .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (02) :725-737
123456