Characterization of monoclonal antibodies against integrin alpha(v)beta(5)

被引：3

作者：

Stuiver, I ^{[1
]}

Smith, JW ^{[1
]}

机构：

[1] LA JOLLA CANC RES FDN,PROGRAM CELL ADHES & EXTRACELLULAR MATRIX,LA JOLLA,CA 92037

来源：

HYBRIDOMA | 1995年 / 14卷 / 06期

关键词：

D O I：

10.1089/hyb.1995.14.545

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A panel of monoclonal antibodies directed against the human integrin alpha(v) beta(5) has been generated, Mice were immunized using human alpha(v) beta(5) purified from placenta. Two hundred hybridoma clones were screened by ELISA and 14 were found to react with purified integrin alpha(v) beta(5). Here we characterize the monoclonal antibodies secreted by three clones, 14H4, 6B9, and 15F11. Two of these antibodies, 6B9 and 15F11, are highly specific for integrin alpha(v) beta(5). These two antibodies do not bind the highly homologous integrin alpha(v) beta(3). The binding of 6B9 and 15F11 to integrin alpha(v) beta(5) is not influenced by ligand nor by divalent cation, Antibody 14H4, obtained in the same fusion, recognizes the alpha(v) subunit.

引用

页码：545 / 550

页数：6

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