INDUCIBLE AMBER SUPPRESSOR FOR BACILLUS-SUBTILIS

被引：7

作者：

GRUNDY, FJ ^{[1
]}

HENKIN, TM ^{[1
]}

机构：

[1] ALBANY MED COLL,DEPT BIOCHEM & MOLEC BIOL,A-10,NEW SCOTLAND AVE,ALBANY,NY 12208

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 07期

关键词：

D O I：

10.1128/jb.176.7.2108-2110.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An amber suppressor variant of Bacillus subtilis tyrosyl-tRNA was constructed and placed under control of the isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible P(spac) promoter. Addition of IPTG resulted in a 50-fold increase in the expression of an rpsD-lacZ fusion containing a UAG amber codon. This system permitted isolation of a conditional lethal mutant which required IPTG for growth.

引用

页码：2108 / 2110

页数：3

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