THE EXPRESSION OF THE MAJOR SHED TRYPANOSOMA-CRUZI ANTIGEN RESULTS FROM THE DEVELOPMENTALLY-REGULATED TRANSCRIPTION OF A SMALL GENE FAMILY

被引：7

作者：

AFFRANCHINO, JL ^{[1
]}

POLLEVICK, GD ^{[1
]}

FRASCH, ACC ^{[1
]}

机构：

[1] FDN CAMPOMAR, INST INVEST BIOQUIM, RA-1405 BUENOS AIRES, ARGENTINA

来源：

FEBS LETTERS | 1991年 / 280卷 / 02期

关键词：

SHED ANTIGEN; GENE FAMILY; TELOMERE; TRYPANOSOMA-CRUZI;

D O I：

10.1016/0014-5793(91)80320-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To better understand the mechanisms involved in the developmental expression of Trypanosoma cruzi antigens we examined the gene structure and transcription properties of the major shed trypomastigote antigen (SAPA). We report in this paper that SAPA is encoded by a small family of at least 6 genes which differ mainly in the length of a repeat region made up of tandemly arranged 36-bp repeat units. SAPA genes are located distant from chromosomal telomeres as inferred from their insensitivity to Bal31 nuclease treatment. Furthermore, Northern blot and S1 protection analyses strongly support the fact that most (or all) SAPA genes are transcribed in the infective form of the parasite.

引用

页码：316 / 320

页数：5

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