EFFECTS OF LIPOXIN-A, ON CHEMOTAXIS AND DEGRANULATION OF HUMAN EOSINOPHILS STIMULATED BY PLATELET-ACTIVATING-FACTOR AND N-FORMYL-L-METHIONYL-L-LEUCYL-L-PHENYLALANINE

被引：86

作者：

SOYOMBO, O ^{[1
]}

SPUR, BW ^{[1
]}

LEE, TH ^{[1
]}

机构：

[1] GUYS HOSP,UMDS,DEPT ALLERGY & ALLIED RESP DISORDERS,LONDON SE1 9RT,ENGLAND

来源：

ALLERGY | 1994年 / 49卷 / 04期

关键词：

ALLERGY; ASTHMA; CHEMOTAXIS; EOSINOPHIL; LEUKOTRIENE; LIPOXIN;

D O I：

10.1111/j.1398-9995.1994.tb02654.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Lipoxins are trihydroxytetraene metabolites derived through a double lipoxygenation of arachidonic acid. Lipoxin A(4) (LXA(4)) was prepared by total chemical synthesis, and its capacity to modulate eosinophil migration has been evaluated. LXA(4) is a weak and partial chemotactic agent; at 10(-6) M, it achieved about 20% of the response of 10(-6) M platelet-activating factor (PAF). Preincubation of eosinophils with increasing doses of LXA(4) (10(-10)-10(-5) M) resulted in a concentration-dependent inhibition of cell migration induced by 10(-6) M formyl-methionyl-leucyl-phenylalanine (FMLP) and 10(-6) M PAF. The concentration of LXA(4) which produced 50% inhibition (IC50) of eosinophil migration was approximately 10(-6) M. LXA(4) (10(-10)-10(-6) M) did not elicit ECP release or modulate ECP release induced by 10(-6) M FMLP. LXA(4) may have antiallergic properties in preventing eosinophilic migration.

引用

页码：230 / 234

页数：5

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