DETECTION OF CRYPTOSPORIDIUM-PARVUM DNA IN FIXED, PARAFFIN-EMBEDDED TISSUE BY THE POLYMERASE CHAIN-REACTION

被引：24

作者：

LAXER, MA ^{[1
]}

DNICUOLA, ME ^{[1
]}

PATEL, RJ ^{[1
]}

机构：

[1] ARMED FORCES INST PATHOL,DEPT INFECT & PARASIT DIS PATHOL,AMER REGISTRY PATHOL,WASHINGTON,DC 20306

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1992年 / 47卷 / 04期

关键词：

D O I：

10.4269/ajtmh.1992.47.450

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The objective of this project was to demonstrate detection of Cryptosporidium parvum DNA in fixed, paraffin-embedded tissue using the polymerase chain reaction (PCR). DNA was purified from six samples of fixed, paraffin-embedded tissue that were histologically positive for C. parvum and used in the PCR. Previously developed oligonucleotide primers specific for C. parvum were used to amplify a 452-base target sequence, and a 20-base synthetic probe labeled with digoxigenin-11-dUTP was used to detect the amplification product by chemiluminescence. All six samples were positive by PCR; negative controls showed no amplification or hybridization. This approach could provide a sensitive and specific method for detection of parasite material in fixed, paraffin-embedded tissue samples, and prove to be of significant value in retrospective studies of archival material.

引用

页码：450 / 455

页数：6

共 19 条

[1] IDENTIFICATION OF TOXOPLASMA-GONDII IN PARAFFIN-EMBEDDED SECTIONS BY THE POLYMERASE CHAIN-REACTION [J].