DETECTION OF CRYPTOSPORIDIUM-PARVUM DNA IN FIXED, PARAFFIN-EMBEDDED TISSUE BY THE POLYMERASE CHAIN-REACTION

被引：24
作者：
LAXER, MA [1 ]
DNICUOLA, ME [1 ]
PATEL, RJ [1 ]
机构：
[1] ARMED FORCES INST PATHOL,DEPT INFECT & PARASIT DIS PATHOL,AMER REGISTRY PATHOL,WASHINGTON,DC 20306
来源：
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 1992年 / 47卷 / 04期
关键词：
D O I：
10.4269/ajtmh.1992.47.450
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
The objective of this project was to demonstrate detection of Cryptosporidium parvum DNA in fixed, paraffin-embedded tissue using the polymerase chain reaction (PCR). DNA was purified from six samples of fixed, paraffin-embedded tissue that were histologically positive for C. parvum and used in the PCR. Previously developed oligonucleotide primers specific for C. parvum were used to amplify a 452-base target sequence, and a 20-base synthetic probe labeled with digoxigenin-11-dUTP was used to detect the amplification product by chemiluminescence. All six samples were positive by PCR; negative controls showed no amplification or hybridization. This approach could provide a sensitive and specific method for detection of parasite material in fixed, paraffin-embedded tissue samples, and prove to be of significant value in retrospective studies of archival material.
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收藏
页码：450 / 455
页数：6
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