MOLECULAR-CLONING OF 2 RECEPTORS FROM RAT-BRAIN WITH HIGH-AFFINITY FOR SALMON-CALCITONIN

被引：103
作者：
ALBRANDT, K
MULL, E
BRADY, EMG
HERICH, J
MOORE, CX
BEAUMONT, K
机构：
[1] Department of Pharmacology, Amylin Pharmaceuticals, Inc., San Diego
来源：
FEBS LETTERS | 1993年 / 325卷 / 03期
关键词：
CALCITONIN; RECEPTOR; CLONING; PCR; EXPRESSION; RAT BRAIN;
D O I：
10.1016/0014-5793(93)81078-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two receptors with high affinity for salmon calcitonin were cloned from the nucleus accumbens region of rat brain. The deduced 479 amino acid sequence of cDNA clone L2175-D20 (designated C1a receptor) is 78% and 66% identical with those reported for human and porcine calcitonin receptors, respectively. Clone U3237-A2 codes for a receptor (designated C1b) that is identical to C1a except fora 37 aminoacid insert in the second extracellular domain. COS-7 cells transfected with either transcript bound [I-125]salmon calcitonin with high affinity (K(d) = 8 pM for C1a; K(d) = 48 pM for C1b) and responded to salmon calcitonin with increases in cAMP. Tissue distribution studies revealed C1a transcript in rat brain, skeletal muscle, kidney and lung, whereas C1b was predominantly found in brain.
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页码：225 / 232
页数：8
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