HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF GLUTATHIONE CONJUGATES .1. ION-PAIR REVERSED-PHASE CHROMATOGRAPHY AND UV DETECTION

被引：6

作者：

TSIKAS, D ^{[1
]}

BRUNNER, G ^{[1
]}

机构：

[1] MED HSCH HANNOVER,DIV GASTROENTEROL & HEPATOL,W-3000 HANNOVER 51,GERMANY

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1992年 / 343卷 / 03期

关键词：

D O I：

10.1007/BF00322384

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the analysis of glutathione (GSH) conjugates derived from cancerogenic xenobiotics and their metabolites is described. The method is based on reversed-phase ion-pair chromatography using tetrabutylammonium hydrogen-sulphate and UV detection at 270 nm. The GSH conjugate, the cysteine conjugate, the corresponding mercapturic acid, and the electrophilic substrate can be assayed simultaneously in a single run. The method was applied to the determination of GSH S-transferase activity in the liver cytosol of rat, rabbit and man towards different electrophilic acceptors. GSH S-transferase activities obtained by this method correlated well with results obtained by previously reported spectrophotometric assay methods. The GSH conjugation of the strong cancerogenic xenobiotic p-nitrobenzylchloride by isolated rat liver hepatocytes was also investigated.

引用

页码：326 / 329

页数：4

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