ISOLATION AND GENETIC-STUDY OF RHO-FLUORO-DL-PHENYLALANINE-RESISTANT MUTANTS OVERPRODUCING BETA-PHENETHYL-ALCOHOL IN SACCHAROMYCES-CEREVISIAE

被引：25

作者：

FUKUDA, K ^{[1
]}

WATANABE, M ^{[1
]}

ASANO, K ^{[1
]}

OUCHI, K ^{[1
]}

TAKASAWA, S ^{[1
]}

机构：

[1] KYOWA HAKKO KOGYO CO LTD,TOKYO RES LABS,MACHIDA,TOKYO 194,JAPAN

来源：

CURRENT GENETICS | 1991年 / 20卷 / 06期

关键词：

YEAST; MUTANT; RHO-FLUORO-DL-PHENYLALANINE; BETA-PHENETHYL-ALCOHOL;

D O I：

10.1007/BF00334770

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

p-Fluoro-DL-phenylalanine (PFP)-resistant mutants which produce a large amount of beta-phenethyl-alcohol, a rose-like flavor component, were isolated from the isogenic strains X2180-1A and X2180-1B of Saccharomyces cerevisiae. Cells of these mutants accumulated phenylalanine and tryptophan more than 3-fold times that of wild-type cells, while they accumulated less than half the tyrosine. The activity of prephenate dehydrogenase (PDG) (EC 1.3.1.12) was markedly decreased while that of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (EC 4.1.2.15) was increased. Genetic analysis revealed that the mutation occurred at the TYR1 locus, encoding PDG, and that the mutated TYR1 gene, tyr1-pfp, caused both PFP resistance and beta-phenethyl-alcohol overproduction. This was supported by molecular genetic studies with cloned tyr1-p DNA.

引用

页码：449 / 452

页数：4

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