BIOCHEMICAL-CHARACTERIZATION OF LEISHMANIA-(VIANNIA)-BRAZILIENSIS AND LEISHMANIA-(VIANNIA)-PERUVIANA BY ISOENZYME ELECTROPHORESIS

被引：39

作者：

ARANA, M

EVANS, DA

ZOLESSI, A

CUENTAS, AL

AREVALO, J

机构：

[1] UNIV PERUANA CAYETANO HEREDIA,INST MED TROP ALEXANDER VONHUMBOLDT,POB 5045,LIMA 100,PERU

[2] UNIV PERUANA CAYETANO HEREDIA,DEPT BIOL,LIMA,PERU

[3] INST NACL SALUD,CTR INVEST HUGO LUMBERAS CRUZ,LIMA,PERU

[4] UNIV PERUANA CAYETANO HEREDIA,DEPT BIOCHEM,LIMA,PERU

[5] UNIV LONDON LONDON SCH HYG & TROP MED,DEPT MED PARASITOL,LONDON WC1E 7HT,ENGLAND

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1990年 / 84卷 / 04期

关键词：

D O I：

10.1016/0035-9203(90)90025-A

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Leishmanial organisms isolated from 24 patients with Andean cutaneous leishmaniais (uta) and from 7 with sylvatic leishmaniasis in both cutaneous and mucosal forms were characterized on the basis of their isoenzyme profiles for 13 enzymes using both cellulose acetate (CA) and thin-layer starch gel (TLS) electrophoretic techniques. Malate dehydrogenase (MDH) after electrophoresis on CA or TLS and mannose phosphate isomerase (MPI) on TLS were the only enzymes of 13 examined which discriminated between the organisms from patients with uta (L. (V.) peruviana) and those with sylvatic leishmaniasis (L. (V.) braziliensis). Mannose phosphate isomerase gave more clear-cut and reproducible discrimination than did MDH on either TLS or CA, and it is suggested that MPI is a reliable enzyme marker that can be used in routine TLS electrophoresis to distinguish between L. (V.) peruviana and L. (V). braziliensis. © 1990, Royal Society of Tropical Medicine and Hygiene. All rights reserved.

引用

页码：526 / 529

页数：4

共 7 条

[1]

ESCOMEL E, 1913, B SOC PATHOL EXOT, V6, P237

[2] THE ISOLATION AND ISOENZYME CHARACTERIZATION OF LEISHMANIA-BRAZILIENSIS SUBSP FROM PATIENTS WITH CUTANEOUS LEISHMANIASIS ACQUIRED IN BELIZE [J].