OCCURRENCE OF NOVEL TYPES OF NITRIC-OXIDE SYNTHASE IN THE SILKWORM, BOMBYX-MORI

被引：30

作者：

CHOI, SK ^{[1
]}

CHOI, HK ^{[1
]}

KADONOOKUDA, K ^{[1
]}

TANIAI, K ^{[1
]}

KATO, Y ^{[1
]}

YAMAMOTO, M ^{[1
]}

CHOWDHURY, S ^{[1
]}

XU, JH ^{[1
]}

MIYANOSHITA, A ^{[1
]}

DEBNATH, NC ^{[1
]}

ASAOKA, A ^{[1
]}

YAMAKAWA, M ^{[1
]}

机构：

[1] NATL INST SERICULTURAL & ENTOMOL SCI,BIOL DEF LAB,TSUKUBA,IBARAKI 305,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 207卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1209

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitric oxide (NO) synthase activity was detected in fat body and the Malpighian tubles of the silkworm, Bombyx mori. Main NO synthase activity in the fat body was Ca2+/calmodulin-dependent, inducible by bacterial lipopolysaccharide (LPS) and required NADPH, FAD, FMN, dithiothreitol (DTT) and tetrahydrobiopterin (BH4) as cofactors for the full expression of the activity. The Malpighian tubles contained two types of NO synthase. One was Ca2+-independent, calmodulin-dependent and constitutive and the other was Ca2+-dependent and constitutive. The former NO synthase required the same cofactors as fat body NO synthase. The activity of Malpighian tuble NO synthases increased dramatically at the end of the last instar period, just prior to spinning. These results indicate that B. mori contains new types of NO synthase, suggesting the wide distribution and different characteristics of this enzyme among vertebrates and invertebrates. (C) 1995 Academic Press. Inc.

引用

页码：452 / 459

页数：8

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