IDENTIFICATION AND PARTIAL-PURIFICATION OF A BACILLUS-THURINGIENSIS CRYIC DELTA-ENDOTOXIN BINDING-PROTEIN FROM SPODOPTERA-LITTORALIS GUT MEMBRANES

被引：31

作者：

SANCHIS, V ^{[1
]}

ELLAR, DJ ^{[1
]}

机构：

[1] UNIV CAMBRIDGE, DEPT BIOCHEM, CAMBRIDGE CB2 1QW, ENGLAND

来源：

FEBS LETTERS | 1993年 / 316卷 / 03期

关键词：

DELTA-ENDOTOXIN; TOXIN RECEPTOR; MEMBRANE PROTEIN; SPODOPTERA-LITTORALIS; BACILLUS-THURINGIENSIS;

D O I：

10.1016/0014-5793(93)81305-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunoblotting experiments were performed using CryIC and CryIA(c) Bacillus thuringiensis delta-endotoxins to detect the presence of specific toxin binding proteins on Spodoptera littoralis brush border membrane vesicles. The CryIC toxin binds two proteins of 40 and 65 kDa and the CryIA(c) binds a protein of 40 kDa. The CryIA(c) toxin also binds faintly to a 120 kDa protein on S. littoralis brush border membrane vesicles as does a polyclonal antiserum raised against a putative CryIA(c) 120 kDa binding protein from Manduca sexta. The 40 kDa CryIC binding protein was partially purified by affinity chromatography and is therefore a strong candidate for in vivo S. littoralis CryIC toxin receptor.

引用

页码：264 / 268

页数：5

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