SEPARATION AND DETERMINATION OF 2,5-DIMETHOXY-4-METHYLAMPHETAMINE ENANTIOMERS IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：20

作者：

GOTO, J

GOTO, N

HIKICHI, A

NAMBARA, T

机构：

[1] Pharmaceutical Institute, Tohoku University, Sendai

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1979年 / 2卷 / 08期

关键词：

D O I：

10.1080/01483917908060129

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for quantitation of 2, 5-dimethoxy-4-methylamphetamine (DOM) enantiomers in plasma by high-performance liquid chromatography (HPLC) is described, d- and l-DOM were readily converted to the amides by condensation with a newly developed chiral reagent, succinimidyl ester of l-a-methoxy-α-methyl-1-naphthaleneacetic acid. The yielded diastereomers were separated on the μPorasil column with cyclohexane/ethyl acetate (3:1) exhibiting satisfactory k’ and R values. The clean-up procedure by use of Sep-pak C18 and carboxymethyl Sephadex LH-20 (CM-LH-20) proved to be effective for determination of the drug in biological fluids by HPLC. The plasma levels of d- and l-DOM after administration of the racemate to the rabbit were determined by the method thus established. © 1979, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：1179 / 1190

页数：12

共 6 条

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[2]

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