CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDY OF GLUCOSE-DEHYDROGENASE FROM THE ARCHAEBACTERIUM THERMOPLASMA-ACIDOPHILUM

被引：4
作者：
BRIGHT, JR
MACKNESS, R
DANSON, MJ
HOUGH, DW
TAYLOR, GL
TOWNER, P
BYROM, D
机构：
[1] UNIV BATH,DEPT BIOCHEM,CLAVERTON DOWN,BATH BA2 7AY,AVON,ENGLAND
[2] ICI BIOL PROD,BILLINGHAM TS23 1LB,CLEVELAND,ENGLAND
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 222卷 / 02期
关键词：
ARCHAEBACTERIA; GLUCOSE DEHYDROGENASE; CRYSTALLOGRAPHY;
D O I：
10.1016/0022-2836(91)90200-P
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Single crystals of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum were obtained using the hanging-drop vapour diffusion method and polyethylene glycol as a precipitant in the presence of NADP+ at pH 5.4. The crystals belong to the hexagonal space group P6122 or P6522, with unit cell dimensions a = b = 121.9 A ̊, c = 229.6 A ̊ and with two molecules in the asymmetric unit. © 1991.
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页码：143 / 144
页数：2
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