CAMP INHIBITS THE INVITRO ASSEMBLY OF MICROTUBULES

被引：17

作者：

GARLAND, DL

机构：

[1] Laboratory of Biochemistry, National Heart, Lung, Blood Institute, Bethesda

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 198卷 / 01期

关键词：

D O I：

10.1016/0003-9861(79)90426-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for assaying microtubule assembly is described. The method utilizes the colchicine binding property of tubulin. This technique was used to study the effect of cyclic AMP on tubulin assembly using 100,000g supernatant and cycle-purified tubulin prepared from porcine brain. Cyclic AMP, in the presence of NaF, inhibited tubulin assembly from 100,000g supernatant but had no effect on cycle-purified tubulin. © 1979.

引用

页码：335 / 337

页数：3

共 24 条

[1] MICROTUBULE ASSEMBLY IN CULTIVATED GREENE MELANOMA CELLS IS STIMULATED BY DIBUTYRYL ADENOSINE 3' - 5'-CYCLIC MONOPHOSPHATE OR CHOLERA TOXIN [J].

DIPASQUALE, AM ;

MCGUIRE, J ;

MOELLMANN, G ;

WASSERMAN, SJ .

JOURNAL OF CELL BIOLOGY, 1976, 71 (03) :735-748

[2] RE-EXAMINATION OF REACTION BETWEEN COLCHICINE AND TUBULIN [J].

GARLAND, D ;

TELLER, DC .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1975, 253 (JUN30) :232-238

[3] KINETICS AND MECHANISM OF COLCHICINE BINDING TO TUBULIN - EVIDENCE FOR LIGAND-INDUCED CONFORMATIONAL CHANGE [J].

GARLAND, DL .

BIOCHEMISTRY, 1978, 17 (20) :4266-4272

[4] BIOCHEMICAL STUDIES ON INVITRO ASSEMBLY AND DISASSEMBLY OF MICROTUBULES [J].

GASKIN, F ;

CANTOR, CR ;

SHELANSKI, ML .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1975, 253 (JUN30) :133-146

[5] CYCLIC ADENOSINE 3'-5'-MONOPHOSPHATE-STIMULATED PHOSPHORYLATION OF ISOLATED NEUROTUBULE SUBUNITS [J].

GOODMAN, DBP ;

RASMUSSEN, H ;

DIBELLA, F ;

GUTHROW, CE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 67 (02) :652-+

[6] NEUROTUBULIN POLYMERIZATION AND PHOSPHORYLATION REACTIONS CATALYZED BY ASSOCIATED PROTEIN KINASE [J].

LETERRIER, JF ;

RAPPAPORT, L ;

NUNEZ, J .

FEBS LETTERS, 1974, 46 (01) :285-288

[7] ADDITION OF COLCHICINE-TUBULIN COMPLEX TO MICROTUBULE ENDS - MECHANISM OF SUBSTOICHIOMETRIC COLCHICINE POISONING [J].

MARGOLIS, RL ;

WILSON, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3466-3470

[8]

NATH K, 1978, P NATL ACAD SCI USA, V75, P319, DOI 10.1073/pnas.75.1.319

[9] CHARACTERIZATION OF MICROTUBULE ASSEMBLY IN PORCINE BRAIN EXTRACTS BY VISCOMETRY [J].

OLMSTED, JB ;

BORISY, GG .

BIOCHEMISTRY, 1973, 12 (21) :4282-4289

[10] SENSITIVE METHOD FOR MEASURING POLYMERIZED AND DEPOLYMERIZED FORMS OF TUBULIN IN TISSUES [J].

PIPELEERS, DG ;

PIPELEERSMARICHAL, MA ;

SHERLINE, P ;

KIPNIS, DM .

JOURNAL OF CELL BIOLOGY, 1977, 74 (02) :341-350

← 1 2 3 →