Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol Delta(22)-desaturase

被引：62
作者：
Kelly, SL
Lamb, DC
Corran, AJ
Baldwin, BC
Parks, LW
Kelly, DE
机构：
[1] JEALOTTS HILL RES STN,ZENECA AGROCHEM,BRACKNELL RG12 6EY,BERKS,ENGLAND
[2] N CAROLINA STATE UNIV,DEPT MICROBIOL,RALEIGH,NC 27695
来源：
FEBS LETTERS | 1995年 / 377卷 / 02期
关键词：
sterol Delta(22)-desaturase; purification; P450; 61;
D O I：
10.1016/0014-5793(95)01342-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
P450 was purified from microsomal fractions of a strain of Saccharomyces cerevisiae which contained detectable P450 despite the disruption of CYP51A1. The P450 had a molecular mass of 58 kDa, similar to P450 51A1, and in a reconstituted assay with rabbit NADPH-P450 reductase and dilauryl phosphotidylcholine exhibited activity for conversion of ergosta-5,7-dienol into ergosterol. N-Terminal amino acid sequencing of the purified protein corresponded to the translated sequence of P450 61 which was recently identified during sequencing of chromosome XIII. This allowed the function of this family of P450 to be identified as sterol Delta(22)-desaturation in the pathway of ergosterol biosynthesis.
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页码：217 / 220
页数：4
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