IMMOBILIZED CATALYTIC ANTIBODIES IN AQUEOUS AND ORGANIC-SOLVENTS

被引：75

作者：

JANDA, KD ^{[1
]}

ASHLEY, JA ^{[1
]}

JONES, TM ^{[1
]}

MCLEOD, DA ^{[1
]}

SCHLOEDER, DM ^{[1
]}

WEINHOUSE, MI ^{[1
]}

机构：

[1] SCRIPPS CLIN & RES FDN,RES INST,DEPT CHEM,LA JOLLA,CA 92037

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1990年 / 112卷 / 24期

关键词：

D O I：

10.1021/ja00180a035

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe the first report of immobilized catalytic antibodies and their behavior in aqueous as well as organic solvents. The results indicate that under aqueous conditions lipase-like catalytic antibodies bound to an inorganic support retain the same activity and stereospecificity they exhibit in free solution. Furthermore, this immobilization imparts added stability in organic solvents. In addition, one abzyme retained a modest activity in 40% aqueous dimethyl sulfoxide. However, attempts to catalyze the reverse reaction (transesterification) under anhydrous conditions were unsuccessful. The use of abzymes as “synthetic reagents” or in “immobilized-abzyme reactors” is suggested. © 1990, American Chemical Society. All rights reserved.

引用

页码：8886 / 8888

页数：3

共 40 条

[11] CATALYTIC ANTIBODIES WITH LIPASE ACTIVITY AND R-SUBSTRATE OR S-SUBSTRATE SELECTIVITY [J].

JANDA, KD ;

BENKOVIC, SJ ;

LERNER, RA .

SCIENCE, 1989, 244 (4903) :437-440

[12] BAIT AND SWITCH STRATEGY FOR OBTAINING CATALYTIC ANTIBODIES WITH ACYL-TRANSFER CAPABILITIES [J].

JANDA, KD ;

WEINHOUSE, MI ;

SCHLOEDER, DM ;

LERNER, RA ;

BENKOVIC, SJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (03) :1274-1275

[13] ANTIBODY CATALYSIS OF BIMOLECULAR AMIDE FORMATION [J].

JANDA, KD ;

LERNER, RA ;

TRAMONTANO, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (14) :4835-4837

[14]

JANDA KD, IN PRESS BIOTECHNOL

[15]

JANDA KD, 1988, SCIENCE, V241, P1199

[16] SULFHYDRYL-DISULFIDE INTERCHANGE [J].

JENSEN, EV .

SCIENCE, 1959, 130 (3385) :1319-1323

[17] ENZYMES IN ORGANIC-SYNTHESIS [J].

JONES, JB .

TETRAHEDRON, 1986, 42 (13) :3351-3403

[18]

KIECHNER G, 1985, J AM CHEM SOC, V24, P7072

[19] ASYMMETRIC TRANSFORMATIONS CATALYZED BY ENZYMES IN ORGANIC-SOLVENTS [J].

KLIBANOV, AM .

ACCOUNTS OF CHEMICAL RESEARCH, 1990, 23 (04) :114-120

[20]

KLIBANOV AM, 1978, BIOCHEM BIOPH RES CO, V83, P1012, DOI 10.1016/0006-291X(78)91496-1

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