EXPRESSION OF SV40 T-ANTIGEN IN LIPOMA CELLS WITH A CHROMOSOMAL TRANSLOCATION T(3-12) IS NOT SUFFICIENT FOR DIRECT IMMORTALIZATION

被引：13

作者：

BELGE, G ^{[1
]}

KAZMIERCZAK, B ^{[1
]}

MEYERBOLTE, K ^{[1
]}

BARTNITZKE, S ^{[1
]}

BULLERDIEK, J ^{[1
]}

机构：

[1] UNIV BREMEN,CTR HUMAN GENET & GENET COUNSELLING,LEOBENER STR ZHG,W-2800 BREMEN 33,GERMANY

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1992年 / 16卷 / 04期

关键词：

D O I：

10.1016/S0309-1651(06)80139-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

12q13-15 changes are the most frequent cytogenetic abnormalities in human tumor cells. To test their biological significance we used an assay based on lipoma cells with a limited in vitro lifetime and this type of chromosomal aberration. Lipoma cells with a reciprocal translocation t(3;12)(q28;q14) were transformed by transfection with a plasmid containing the SV40 "early region". The transformed cells showed an altered morphology with loss of contact inhibition, formation of foci, and T-antigen expression. They were immortalized after a growth crisis. The karyotypic patterns before and after the crisis show that the translocation together with expression of SV40 T-antigen is not sufficient for direct immortalization. © 1992 Academic Press Ltd.

引用

页码：339 / 347

页数：9

共 21 条

[1] REARRANGEMENTS OF CHROMOSOME REGION 12Q13-]Q15 IN PLEOMORPHIC ADENOMAS OF THE HUMAN SALIVARY-GLAND (PSA) [J].

BULLERDIEK, J ;

BARTNITZKE, S ;

WEINBERG, M ;

CHILLA, R ;

HAUBRICH, J ;

SCHLOOT, W .

CYTOGENETICS AND CELL GENETICS, 1987, 45 (3-4) :187-190

[2] ABERRATIONS OF CHROMOSOME-8 IN MIXED SALIVARY-GLAND TUMORS - CYTOGENETIC FINDINGS ON 7 CASES [J].

BULLERDIEK, J ;

BOSCHEN, C ;

BARTNITZKE, S .

CANCER GENETICS AND CYTOGENETICS, 1987, 24 (02) :205-212

[3] SV 40-INDUCED TRANSFORMATION OF HUMAN DIPLOID CELLS - CRISIS AND RECOVERY [J].

GIRARDI, AJ ;

JENSEN, FC ;

KOPROWSKI, H .

JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1965, 65 (01) :69-+

[4] NEW TECHNIQUE FOR ASSAY OF INFECTIVITY OF HUMAN ADENOVIRUS 5 DNA [J].

GRAHAM, FL ;

VANDEREB, AJ .

VIROLOGY, 1973, 52 (02) :456-467

[5] TRANSFECTION OF LIPOMA CELLS WITH PAPILLOMA BOVINE VIRUS SUBGENOMIC FRAGMENT [J].

HAUS, O ;

MOISANT, M ;

RACINE, L ;

ZAHND, JP ;

PADIEU, P ;

TURCCAREL, C .

CELL BIOLOGY INTERNATIONAL REPORTS, 1991, 15 (01) :67-74

[6] RECIPROCAL TRANSLOCATION T(3-12)(Q27-Q13) IN LIPOMA [J].

HEIM, S ;

MANDAHL, N ;

KRISTOFFERSSON, U ;

MITELMAN, F ;

ROOSER, B ;

RYDHOLM, A ;

WILLEN, H .

CANCER GENETICS AND CYTOGENETICS, 1986, 23 (04) :301-304

[7] DIFFERENT KARYOTYPIC FEATURES CHARACTERIZE DIFFERENT CLINICOPATHOLOGIC SUBGROUPS OF BENIGN LIPOGENIC TUMORS [J].

HEIM, S ;

MANDAHL, N ;

RYDHOLM, A ;

WILLEN, H ;

MITELMAN, F .

INTERNATIONAL JOURNAL OF CANCER, 1988, 42 (06) :863-867

[8] A SPECIFIC TRANSLOCATION, T(12,14)(Q14-15,Q23-24), CHARACTERIZES A SUBGROUP OF UTERINE LEIOMYOMAS [J].

HEIM, S ;

NILBERT, M ;

VANNI, R ;

FLODERUS, UM ;

MANDAHL, N ;

LIEDGREN, S ;

LECCA, U ;

MITELMAN, F .

CANCER GENETICS AND CYTOGENETICS, 1988, 32 (01) :13-17

[9] INVITRO TRANSFORMATION BY THE SV40 EARLY REGION OF CELLS FROM A HUMAN BENIGN SALIVARY-GLAND TUMOR WITH A 12Q13-]Q15 REARRANGEMENT [J].

KAZMIERCZAK, B ;

BARTNITZKE, S ;

HARTL, M ;

BULLERDIEK, J .

CYTOGENETICS AND CELL GENETICS, 1990, 53 (01) :37-39

[10] RAPID SUBCHROMOSOMAL LOCALIZATION OF COSMIDS BY NONRADIOACTIVE INSITU HYBRIDIZATION [J].

KIEVITS, T ;

DAUWERSE, JG ;

WIEGANT, J ;

DEVILEE, P ;

BREUNING, MH ;

CORNELISSE, CJ ;

VANOMMEN, GJB ;

PEARSON, PL .

CYTOGENETICS AND CELL GENETICS, 1990, 53 (2-3) :134-&

← 1 2 3 →