FROM ADENYLATE-CYCLASE TO GUANYLATE-CYCLASE - MUTATIONAL ANALYSIS OF A CHANGE IN SUBSTRATE-SPECIFICITY

被引：20

作者：

BEUVE, A ^{[1
]}

DANCHIN, A ^{[1
]}

机构：

[1] INST PASTEUR,28 RUE DOCTEUR ROUX,F-75724 PARIS 15,FRANCE

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 225卷 / 04期

关键词：

ADENYLATE CYCLASE; GUANYLATE CYCLASE; SUBSTRATE SPECIFICITY; BETA-GALACTOSIDASE FUSION; RHIZOBIUM-MELILOTI;

D O I：

10.1016/0022-2836(92)90093-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adenylate and guanylate cyclases, having different but related substrates, are a paradigm for the study of substrate discrimination. A prokaryotic adenylate cyclase gene, phylogenetically related to eukaryotic counterparts, was screened for mutants remodelling the enzyme's specificity. In a first step, a mutant was selected displaying a significant level of guanylate cyclase activity. This was due to a point mutation destroying most of the adenylate cyclase activity. A second selection step restored most of the original activity. This resulted from an additional mutation in the same region, thus permitting the first identification of a functional domain in adenylate and guanylate cyclases. © 1992.

引用

页码：933 / 938

页数：6

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