CLONING, NUCLEOTIDE-SEQUENCE, AND ENGINEERED EXPRESSION OF THERMUS-THERMOPHILUS DNA-LIGASE, A HOMOLOG OF ESCHERICHIA-COLI DNA-LIGASE

被引：47
|
作者：
LAUER, G
RUDD, EA
MCKAY, DL
ALLY, A
ALLY, D
BACKMAN, KC
机构：
[1] BIOTECHNICA INT INC, 85 BOLTON ST, CAMBRIDGE, MA 02140 USA
[2] BIOTECHNICA DIAGNOST INC, CAMBRIDGE, MA 02138 USA
来源：
JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 16期
关键词：
D O I：
10.1128/jb.173.16.5047-5053.1991
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We have cloned and sequenced the gene for DNA ligase from Thermus thermophilus. A comparison of this sequence and those of other ligases reveals significant homology only with that of Escherichia coli. The overall amino acid composition of the thermophilic ligase and the pattern of amino acid substitutions between the two proteins are consistent with compositional biases in other thermophilic enzymes. We have engineered the expression of the T. thermophilus gene in Escherichia coli, and we show that E. coli proteins may be substantially removed from the thermostable ligase by a simple heat precipitation step.
引用
收藏
页码：5047 / 5053
页数：7
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