AN ALTERNATIVE PROTOCOL FOR THE SERIAL PASSAGE AND MAINTENANCE OF A HOMOGENIC STRAIN OF STRONGYLOIDES-RATTI IN FEMALE SPRAGUE-DAWLEY RATS

被引：0

作者：

MENDIS, AHW

GRUBB, WB

ARMSON, A

机构：

[1] School of Biomedical Sciences, Curtin University of Technology, Perth, 6001 WA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1993年 / 23卷 / 06期

关键词：

STRONGYLOIDES-RATTI; IN-VITRO CULTURE; MURINE MODEL OF STRONGYLOIDIASIS;

D O I：

10.1016/0020-7519(93)90080-I

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A method which does not involve the tedious use of watch glass coprocultures for obtaining filariform infective (L3) larvae of Strongyloides ratti from faecal pellets of infected Sprague-Dawley rats is described. The alternative method utilises Baermannization (18 h) of faecal pellets to yield rhabditiform (L1) larvae of S. ratti and their subsequent culture for 72 h at 19-degrees-C in tissue-culture-flasks containing only dechlorinated tap water to yield infective filariform (L3) larvae. The yields and infectivity of the L3 larvae obtained from the two methods were essentially similar.

引用

页码：813 / 814

页数：2

共 5 条

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