A MODIFIED ASSAY FOR BLOOD-COAGULATION FACTOR-XIII USING ENZYME IMMOBILIZATION ON STAPHYLOCOCCUS-AUREUS - PRELIMINARY EVALUATION

被引：1

作者：

WHYARD, TC ^{[1
]}

ABLIN, RJ ^{[1
]}

机构：

[1] SUNY STONY BROOK,HLTH SCI CTR,DEPT UROL,IMMUNOL UNIT,STONY BROOK,NY 11794

来源：

FIBRINOLYSIS | 1991年 / 5卷 / 02期

关键词：

ENZYME IMMOBILIZATION; FACTOR-XIII; PROTEIN-A (STAPHYLOCOCCUS-AUREUS); TRANSGLUTAMINASE;

D O I：

10.1016/0268-9499(91)90055-9

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The binding of anti-factor (F) XIII antibody to protein A on Staphylococcus aureus (Staph Aureus) was utilised to selectively remove FXIII (a protransglutaminase) from the sample. Following binding by specific antibody, the presence of the transglutaminase was then evaluated by a filter paper assay. Used in conjunction with the filter paper assay, preliminary evaluation of the Staph Aureus assay appears to provide increased sensitivity for quantification of FXIII activity in fibrinogen containing samples over that of the filter paper assay alone. In addition, the modified assay circumvents the requirement to remove or neutralise endogenous fibrinogen (in applicable samples).

引用

页码：127 / 130

页数：4

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