EXPRESSION OF ESCHERICHIA-COLI RIBOSOMAL-PROTEIN AND RNA-POLYMERASE GENES CLONED ON PLASMIDS

被引：74
作者：
FIIL, NP
BENDIAK, D
COLLINS, J
FRIESEN, JD
机构：
[1] YORK UNIV,DEPT BIOL,DOWNSVIEW M3J 1P3,ONTARIO,CANADA
[2] UNIV COPENHAGEN,INST MICROBIOL,DK-1353 COPENHAGEN K,DENMARK
[3] GESELL BIOTECHNOL FORSCH MBH,GENET ABT,D-3300 BRUNSWICK,FED REP GER
来源：
MOLECULAR & GENERAL GENETICS | 1979年 / 173卷 / 01期
关键词：
D O I：
10.1007/BF00267689
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fragments of λdrifd 18 DNA with different end-points within the set of structural genes of ribosomal proteins L11 (rplK), L1 (rplA), L10 (rplJ) and L12 (rplL) as well as the β (rpoB) and β′ (rpoC) subunits of RNA polymerase have been cloned on plasmids. These plasmids were transformed in host cells which were mutant for each of the genes, enabling expression of both wild-type (plasmid-borne) and mutant (chromosomal) genes to be differentiated. On the basis of these results we propose the following genetic structure for the region: rplK and rplA are in one operon; rplL, rpoB and rpoC are in a second. Our data suggest the possibility that rplJ is by itself in an operon situated between the other two. © 1979 Springer-Verlag.
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页码：39 / 50
页数：12
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