N-6-ADENOSINE METHYLATION IN MESSENGER-RNA - SUBSTRATE-SPECIFICITY AND ENZYME COMPLEXITY

被引：41

作者：

ROTTMAN, FM ^{[1
]}

BOKAR, JA ^{[1
]}

NARAYAN, P ^{[1
]}

SHAMBAUGH, ME ^{[1
]}

LUDWICZAK, R ^{[1
]}

机构：

[1] CASE WESTERN RESERVE UNIV,SCH MED,DEPT INTERNAL MED,CLEVELAND,OH 44106

来源：

BIOCHIMIE | 1994年 / 76卷 / 12期

关键词：

MESSENGER-RNA METHYLATION; N-6-ADENOSINE; METHYLTRANSFERASE;

D O I：

10.1016/0300-9084(94)90038-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The N-6-methylation of internal adenosine residues is a common post-transcriptional modification of eukaryotic pre-mRNA sequences. An in vitro methylation system which retains the precise selectivity of in vivo methylation sites has been used to further define the nature of RNA site recognition. In addition to short consensus sequences, other structural features or context effects contribute to the selection of methylation sites in pre-mRNAs. Partial purification of the mRNA N-6-adenosine methyltransferase revealed unexpected levels of complexity. The methyltransferase is composed of three separate components with molecular masses of 30, 200 and 875 kDa, respectively. These components are readily separated under non-denaturing conditions and each is required for mRNA methylation activity.

引用

页码：1109 / 1114

页数：6

共 27 条

[1] LOCALIZATION OF N6-METHYLADENOSINE IN ROUS-SARCOMA VIRUS GENOME
BEEMON, K
KEITH, J
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1977, 113 (01) : 165 - 179
[2] BOKAR JA, 1994, J BIOL CHEM, V269, P17697
[3] N-6-METHYLADENOSINE RESIDUES IN AN INTRON-SPECIFIC REGION OF PROLACTIN PRE-MESSENGER-RNA
CARROLL, SM
NARAYAN, P
ROTTMAN, FM
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (09) : 4456 - 4465
[4] CSEPANY T, 1990, J BIOL CHEM, V265, P20117
[5] CHARACTERIZATION OF NOVIKOFF HEPATOMA MESSENGER-RNA METHYLATION AND HETEROGENEITY IN METHYLATED 5' TERMINUS
DESROSIERS, RC
FRIDERICI, KH
ROTTMAN, FM
[J]. BIOCHEMISTRY, 1975, 14 (20) : 4367 - 4374
[6] 5' TERMINAL NUCLEOTIDE-SEQUENCES OF MESSENGER-RNAS OF DICTYOSTELIUM DISCOIDEUM
DOTTIN, RP
WEINER, AM
LODISH, HF
[J]. CELL, 1976, 8 (02) : 233 - 244
[7] EICHLER DC, 1987, BIOCHEMISTRY-US, V26, P1629
[8] ENSINGER MJ, 1976, J BIOL CHEM, V251, P5283
[9] METHYLATIONS OF ADENOSINE RESIDUES (M6A) IN PRE-MESSENGER RNA ARE IMPORTANT FOR FORMATION OF LATE SIMIAN VIRUS-40 MESSENGER-RNAS
FINKEL, D
GRONER, Y
[J]. VIROLOGY, 1983, 131 (02) : 409 - 425
[10] SEQUENCE SPECIFICITY OF THE HUMAN MESSENGER-RNA N6-ADENOSINE METHYLASE INVITRO
HARPER, JE
MICELI, SM
ROBERTS, RJ
MANLEY, JL
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (19) : 5735 - 5741

← 1 2 3 →