A METHOD FOR PREPARATION OF RENAL TUBULAR BASEMENT-MEMBRANE

被引：10
作者：
BUTKOWSKI, RJ
HAGEN, S
机构：
[1] Department of Laboratory Medicine and Pathology, University of MN, Minneapolis, MN
来源：
CONNECTIVE TISSUE RESEARCH | 1990年 / 25卷 / 02期
关键词：
Basement membrane; Kidney;
D O I：
10.3109/03008209009006986
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A method is presented for the preparation of tubular basement membrane from renal cortex. The procedure utilizes a Polytron tissue disrupter, differential sieving, and for some species, sucrose density centrifugation. The procedure is especially useful for obtaining highly purified rabbit and bovine tubular basement membranes. Human tubular and glomerular basement membranes can be prepared with some cross contamination. The method offers the capability of rapidly generating large quantities of the basement membranes that retain the Goodpasture antigen and a TBM antigen associated with anti-TBM nephritis. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
引用
收藏
页码：121 / 130
页数：10
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