HPLC IN PHARMACOKINETICS AND METABOLISM STUDIES

The use of high-performance liquid chromatography in kinetics and metabolism studies is demonstrated. The following kinetic parameters are ascertained after single oral and intravenous administration of a drug: absorption by areal comparison, elimination constant in the β-phase, and clearance. Concentration levels in serum are measured after repeated oral administration on several days, with pharmacokinetic model fit to estimate limit concentrations in the steady state. Species-dependencies in metabolism are investigated in 24-h urines. The necessary characterization of drug and metabolite peaks is obtained by in-series connection of several detectors. The relative bioavailability of a controlled release form is determined by measuring drug concentrations in the blood. © 1979 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.