ISOLATION OF STABLE AGGREGATES OF IGG BY ZONAL ULTRA-CENTRIFUGATION IN SUCROSE GRADIENTS CONTAINING ALBUMIN

被引：44

作者：

KNUTSON, DW

KIJLSTRA, A

LENTZ, H

VANES, LA

机构：

[1] VET ADM WADSWORTH HOSP CTR,MED RES LABS,LOS ANGELES,CA 90073

[2] UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024

[3] STATE UNIV LEIDEN HOSP,DEPT NEPHROL,LEIDEN,NETHERLANDS

来源：

IMMUNOLOGICAL COMMUNICATIONS | 1979年 / 8卷 / 03期

关键词：

D O I：

10.3109/08820137909050047

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Soluble heat aggregates of [125I]IgG (A-IgG) were prepared and separated by gel filtration or by zonal ultracentrifugation, and fractions containing different size aggregates were then analyzed by reultracentrifugation. A-IgG formed single narrow peaks with constant S rates when isolated, stored and analyzed in solution containing 0.5% serum albumin. Thus, stable homogeneous aggregates of IgG of known size can be simply prepared and should prove useful both as a model for immune complexes of specified sizes and as a standard for immune complex assays. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：337 / 345

页数：9

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