ENHANCEMENT OF BOVINE PANCREATIC RIBONUCLEASE-ACTIVITY BY MERCAPTOETHANOL

被引：9

作者：

WATKINS, JB

BENZ, FW

机构：

来源：

SCIENCE | 1978年 / 199卷 / 4333期

关键词：

D O I：

10.1126/science.564548

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Incubation of ribonuclease with 0.1 M mercaptoethanol at pH 8.5 can increase the enzyme's hydrolytic activity toward cytidine 2′,3′- monophosphate (cyclic CMP) under standard assay conditions. Cation-exchange chromatography of the ribonuclease-thiol reaction mixture revealed seven fractions. The fraction with the highest activity had an approximate tenfold decrease in the apparent Michaelis constant for cyclic CMP with respect to native ribonuclease. The enhanced activity is a metastable property since this fraction reverts back to the control activity and chromatographic behavior ofnative ribonuclease on standing in solution at room temperature.

引用

页码：1084 / 1087

页数：4

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