A GROWTH-RELATED MESSENGER-RNA IN CULTURED MOUSE CELLS ENCODES A PLACENTAL CALCIUM-BINDING PROTEIN

被引：108
作者：
JACKSONGRUSBY, LL
SWIERGIEL, J
LINZER, DIH
机构：
[1] Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston
来源：
NUCLEIC ACIDS RESEARCH | 1987年 / 15卷 / 16期
关键词：
D O I：
10.1093/nar/15.16.6677
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have characterized an mRNA that increases in abundance after serum stimulation of quiescent mouse fibroblasts. This mRNA, designated 18A2, encodes a predicted polypeptide of 101 amino acids with homology to known calcium binding proteins. A variety of mouse tissues express the 18A2 mRNA, with the highest levels detected in the non-pregnant uterus and in the placenta. The concentration of 18A2 mRNA in total placental RNA decreases from day 8 to day 10 of pregnancy, and is below detection throughout the latter half of gestation. In serum-stimulated fibroblasts, the increase in 18A2 mRNA is dependent on protein synthesis. The 18A2 mRNA is similar in size, serum-inducibility, and sequence to the 2A9 mRNA (1), but these mRNAs are derived from distinct genes. This suggests that the mouse genome harbors a family of serum-inducible genes encoding proteins predicted to bind calcium. © 1987 IRL Press Limited, Oxford, England.
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页码：6677 / 6690
页数：14
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