PURIFICATION OF CLONED AND GENOMIC DNA BY GUANIDINE THIOCYANATE ISOBUTYL ALCOHOL FRACTIONATION

被引：39
作者：
NELSON, JE
KRAWETZ, SA
机构：
[1] WAYNE STATE UNIV,SCH MED,CS MOTT CTR HUMAN GROWTH & DEV,DEPT OBSTET & GYNECOL,DETROIT,MI 48201
[2] WAYNE STATE UNIV,SCH MED,CS MOTT CTR HUMAN GROWTH & DEV,DEPT MOLEC BIOL & GENET,DETROIT,MI 48201
来源：
ANALYTICAL BIOCHEMISTRY | 1992年 / 207卷 / 01期
关键词：
D O I：
10.1016/0003-2697(92)90523-A
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a rapid and efficient method to purify cloned or high molecular weight eukaryotic DNA from cell lysates using guanidine thiocyanate and isobutyl alcohol. This simple two-step extraction procedure utilizes isobutyl alcohol to fractionate DNA away from cell lysates containing guanidine thiocyanate, yielding a relatively large quantity of pure DNA. The yield of DNA is approximately twice that obtained by the organic extraction method. DNA purified with this chaotropic-based protocol is suitable for a variety of applications including restriction analysis, double-stranded sequencing, PCR, subcloning, and transcription. © 1992.
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页码：197 / 201
页数：5
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