GENE PROBE ANALYSIS OF SOIL MICROBIAL-POPULATIONS SELECTED BY AMENDMENT WITH 2,4-DICHLOROPHENOXYACETIC ACID

被引:100
作者
HOLBEN, WE
SCHROETER, BM
CALABRESE, VGM
OLSEN, RH
KUKOR, JK
BIEDERBECK, VO
SMITH, AE
TIEDJE, JM
机构
[1] MICHIGAN STATE UNIV,DEPT CROP & SOIL SCI,E LANSING,MI 48824
[2] W VIRGINIA UNIV,DEPT PLANT PATHOL & ENVIRON MICROBIOL,MORGANTOWN,WV 26506
[3] UNIV MICHIGAN,SCH MED,DEPT MICROBIOL & IMMUNOL,ANN ARBOR,MI 48109
[4] AGR CANADA,RES STN,SWIFT CURRENT S9H 3X2,SASKATCHEWAN,CANADA
[5] AGR CANADA,RES STN,REGINA S4P 3A2,SASKATCHEWAN,CANADA
关键词
D O I
10.1128/AEM.58.12.3941-3948.1992
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Soils with a history of 2,4-dichlorophenoxyacetic acid (2,4-D) treatment at field application rates and control soils with no prior exposure to 2,4-D were amended with 2,4-D in the laboratory. Before and during these treatments, the populations of 2,4-D-degrading bacteria were monitored by most-probable-number (MPN) enumeration and hybridization analyses, using probes for the tfd genes of plasmid pJP4, which encode enzymes for 2,4-D degradation. Data obtained by these alternate methods were compared. Several months after the most recent field application of 2,4-D (approximately 1 ppm), soils with a 42-year history of 2,4-D treatment did not have significantly higher numbers of 2,4-D-degrading organisms than did control soils with no prior history of treatment. In response to laboratory amendments with 2,4-D, both the previously treated soils and those with no prior history of exposure exhibited a dramatic increase in the number of 2,4-D-metabolizing organisms. The MPN data indicate a 4- to 5-log population increase after one amendment with 250 ppm of 2,4-D and ultimately a 6- to 7-log increase after four additional amendments, each with 400 ppm of 2,4-D. Similarly, when total bacterial DNA from the soil microbial community of these samples was analyzed by using a probe for the tfdA gene (2,4-D monooxygenase) or the tfdB gene (2,4-dichlorophenol hydroxylase) a dramatic increase in the level of hybridization was observed in both soils. Probes to the tfdC, -D, -E, and -F genes did not hybridize to the bacterial community DNA to any significant extent before or after 2,4-D treatment, indicating that pathways different from the canonical pJP4-encoded pathway at the DNA sequence level, and possibly at the functional level, account for the degradative activity in these soils. Quantitative hybridization data and MPN values were in agreement, indicating that most of the 2,4-D-degrading populations were detected by the tfdA and tfdB gene probes. The hybridization patterns detected in Southern analyses of bacterial community DNA indicated that a dominant 2,4-D-degrading population was selected and maintained in these soils.
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页码:3941 / 3948
页数:8
相关论文
共 36 条
[1]  
Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210
[2]   PREPARATION OF A DNA GENE PROBE FOR DETECTION OF MERCURY RESISTANCE GENES IN GRAM-NEGATIVE BACTERIAL COMMUNITIES [J].
BARKAY, T ;
FOUTS, DL ;
OLSON, BH .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 49 (03) :686-692
[3]   PHENOTYPIC AND GENOTYPIC ADAPTATION OF AEROBIC HETEROTROPHIC SEDIMENT BACTERIAL COMMUNITIES TO MERCURY STRESS [J].
BARKAY, T ;
OLSON, BH .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1986, 52 (02) :403-406
[4]   EFFECTS OF LONG-TERM 2,4-D FIELD APPLICATIONS ON SOIL BIOCHEMICAL PROCESSES [J].
BIEDERBECK, VO ;
CAMPBELL, CA ;
SMITH, AE .
JOURNAL OF ENVIRONMENTAL QUALITY, 1987, 16 (03) :257-262
[5]   COMPARATIVE GENETIC ORGANIZATION OF INCOMPATIBILITY GROUP-P DEGRADATIVE PLASMIDS [J].
BURLAGE, RS ;
BEMIS, LA ;
LAYTON, AC ;
SAYLER, GS ;
LARIMER, F .
JOURNAL OF BACTERIOLOGY, 1990, 172 (12) :6818-6825
[6]  
CALABRESE VGM, 1991, 83RD ANN M AM SOC AG, P260
[7]  
CALABRESE VGM, 1992, 92 GEN M AM SOC MICR, P341
[8]   THE ENUMERATION OF 2,4-D DEGRADERS IN SASKATCHEWAN SOILS [J].
CULLIMORE, DR .
WEED SCIENCE, 1981, 29 (04) :440-443
[9]   PROPERTIES OF 6 PESTICIDE DEGRADATION PLASMIDS ISOLATED FROM ALCALIGENES-PARADOXUS AND ALCALIGENES-EUTROPHUS [J].
DON, RH ;
PEMBERTON, JM .
JOURNAL OF BACTERIOLOGY, 1981, 145 (02) :681-686
[10]   TRANSPOSON MUTAGENESIS AND CLONING ANALYSIS OF THE PATHWAYS FOR DEGRADATION OF 2,4-DICHLOROPHENOXYACETIC ACID AND 3-CHLOROBENZOATE IN ALCALIGENES-EUTROPHUS JMP134(PJP4) [J].
DON, RH ;
WEIGHTMAN, AJ ;
KNACKMUSS, HJ ;
TIMMIS, KN .
JOURNAL OF BACTERIOLOGY, 1985, 161 (01) :85-90