RADICAL INTERMEDIATES IN VERATRYL ALCOHOL OXIDATION BY LIGNINASE - NMR EVIDENCE

被引：33
作者：
GILARDI, G
HARVEY, PJ
CASS, AEG
PALMER, JM
机构：
[1] IMPERIAL COLL SCI TECHNOL & MED,CTR BIOTECHNOL,LONDON SW7 2AZ,ENGLAND
[2] IMPERIAL COLL SCI TECHNOL & MED,DEPT PURE & APPL BIOL,LONDON SW7 2AZ,ENGLAND
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1041卷 / 02期
关键词：
(P. chrysosporium); Lignin; Ligninase; NMR; Veratryl alcohol;
D O I：
10.1016/0167-4838(90)90055-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Proton nuclear magnetic resonance (NMR) spectra of veratryl alcohol (3,4-dimethoxybenzyl alcohol) were obtained during its oxidation by ligninase. It was observed that a substantial increase in the linewidths of the resonances occurred only in the presence of both the enzyme and hydrogen peroxide. Quenching the reaction by the addition of alkali immediately restored the normal linewidths of the resonances. Furthermore, inversion-recovery experiments showed a decrease in the longitudinal relaxation time of the substrate when the enzyme was actively turning over. Changes in both these NMR parameters are consistent with the generation of radical intermediates during the ligninase-catalysed oxidation of veratryl alcohol. © 1990.
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页码：129 / 132
页数：4
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