DETERMINATION OF D-PENICILLAMINE IN SERUM AND URINE OF PATIENTS WITH RHEUMATOID-ARTHRITIS

被引：36

作者：

MUIJSERS, AO ^{[1
]}

VANDESTADT, RJ ^{[1
]}

HENRICHS, AMA ^{[1
]}

VANDERKORST, JK ^{[1
]}

机构：

[1] AMSTERDAM CTR RHEUMAT DIS,NL-1056 AB AMSTERDAM,NETHERLANDS

来源：

CLINICA CHIMICA ACTA | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1016/0009-8981(79)90010-X

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A Chromatographic auto-analyser method is described for the determination of total D-penicillamine in biological fluids. After oxidation with performic acid, D-penicillaminic acid is separated from other ninhydrin-positive acidic amino acids by anion-exchange chromatography. Total cysteine/cystine is simultaneously determined as cysteic acid, which makes the method equally suitable for patients with cystinuria. The detection limit in serum and urine is 2 μM D-penicillamine, which is more than sufficient for clinical application. The metabolite S-methyl-D-penicillamine is oxidized by performic acid to a sulphone, which can be determined after separation by cation-exchange chromatography. © 1979.

引用

页码：173 / 180

页数：8

共 22 条

[1] STRUCTURE, PROPERTIES, AND FUNCTION OF A COPPER(I)-COPPER(II) COMPLEX OF D-PENICILLAMINE - PENTATHALLIUM(I) MU-8-CHLORO-DODECA(D-PENICILLAMINATO)-OCTACUPRATE(I)HEXACUPRATE(II) NORMAL-HYDRATE [J].

BIRKER, PJMWL ;

FREEMAN, HC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1977, 99 (21) :6890-6899

[2] BESTIMMUNG VON PENICILLAMIN ALS PENICILLAMINSAURE DURCH CHROMATOGRAPHIE UBER ANIONENAUSTAUSCHER [J].

BORNER, K .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1965, 341 (4-6) :264-&

[3]

EKBERG M, 1974, ACTA MED SCAND, V195, P415

[4]

ELDJARN L, 1964, SCAND J CLIN LAB INV, V16, P153

[5] CYSTEAMINE, PENICILLAMINE, GLUTATHIONE, AND THEIR DERIVATIVES ANALYZED BY AUTOMATED ION-EXCHANGE COLUMN CHROMATOGRAPHY [J].

HSIUNG, M ;

YEO, YY ;

ITIABA, K ;

CRAWHALL, JC .

BIOCHEMICAL MEDICINE, 1978, 19 (03) :305-317

[6]

Jaffe I.A., 1976, PENICILLAMINE RES RH, P11

[7] QUANTITATIVE DETERMINATION OF BIOLOGICALLY IMPORTANT THIOLS AND DISULFIDES BY GAS-LIQUID CHROMATOGRAPHY [J].

JELLUM, E ;

BACON, VA ;

PATTON, W ;

PEREIRA, W ;

HALPERN, B .

ANALYTICAL BIOCHEMISTRY, 1969, 31 (1-3) :339-&

[8] ION-EXCHANGE CHROMATOGRAPHY OF PHYSIOLOGICAL SULFUR AMINO-ACIDS ON A HIGHLY CROSSLINKED RESIN [J].

JEPPSSON, JO ;

KARLSSON, IM .

JOURNAL OF CHROMATOGRAPHY, 1972, 72 (01) :93-&

[9]

LENGFELDER E, 1978, H-S Z PHYSIOL CHEM, V359, P751

[10]

LYLE WH, 1977, P ROY SOC MED S3, V70

← 1 2 3 →