DETERMINATION OF FREE AMINO-ACID ENANTIOMERS IN RAT-BRAIN AND SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER DERIVATIZATION WITH N-TERT-BUTYLOXYCARBONYL-L-CYSTEINE AND O-PHTHALDIALDEHYDE

被引：273

作者：

HASHIMOTO, A ^{[1
]}

NISHIKAWA, T ^{[1
]}

OKA, T ^{[1
]}

TAKAHASHI, K ^{[1
]}

HAYASHI, T ^{[1
]}

机构：

[1] NCNP,NATL INST NEUROSCI,DIV BIOCHEM & CELLULAR BIOL,KODAIRA,TOKYO 187,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 582卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(92)80300-F

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The concurrent determination of free amino acid enantiomers and non-chiral amino acids in rat brain and serum was accomplished by high-performance liquid chromatography with fluorimetric detection after derivatization with N-tert.-butyloxycarbonyl-L-cysteine and o-phthaldialdehyde. The method revealed the presence of a large amount of free D-serine (0.22 mumol/g of tissue; D/D + L ratio = 0.25) in the brain whereas D-aspartate and D-alanine were established to be at trace levels. These results further support the presence of D-serine in adult brain tissues as demonstrated by recent work using gas chromatography.

引用

页码：41 / 48

页数：8

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