SIGNAL TRANSDUCTION AND PHARMACOLOGICAL CHARACTERISTICS OF A METABOTROPIC GLUTAMATE RECEPTOR, MGLUR1, IN TRANSFECTED CHO CELLS

被引：549
作者：
ARAMORI, I
NAKANISHI, S
机构：
[1] Institute for Immunology Kyoto University Faculty of Medicine Kyoto
来源：
NEURON | 1992年 / 8卷 / 04期
关键词：
D O I：
10.1016/0896-6273(92)90096-V
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The signal transduction and pharmacological properties of a metabotropic glutamate receptor, mGluR1, were studied in CHO cells permanently expressing the cloned receptor. mGluR1 stimulated phosphatidylinositol (PI) hydrolysis in the potency rank order of quisqualate > L-glutamate greater-than-or-equal-to ibotenate > L-homocysteine sulfinate greater-than-or-equal-to trans-ACPD. This receptor also evoked the stimulation of cAMP formation and arachidonic acid release with comparable agonist potencies. DL-AP3 and L-AP4, the effective antagonists reported for glutamate-stimulated PI hydrolysis in brain slices, showed no appreciable effects on mGluR1, suggesting the existence of an additional subtype of this receptor family. Pertussis toxin and phorbol ester produced distinct effects on the three transduction cascades, implying that mGluR1 independently links to the multiple transduction pathways probably through different G proteins.
引用
收藏
页码：757 / 765
页数：9
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