SEPARATION AND ESTIMATION OF SMALL AMOUNTS OF THE ENANTIOMERS OF CARBIDOPA AND METHYLDOPA ON A CHIRAL STATIONARY PHASE WITH L-PHENYLALANINE AS SELECTOR IN LIGAND-EXCHANGE CHROMATOGRAPHY

The chiral stationary phase with L-phenylalanine as selector was used for the separation of the enantiomers of carbidopa and methyldopa in ligand-exchange chromatography. The influence of pH, organic modifier and copper(II) salt concentration in the eluent on capacity and separation factors has been shown. The applicability of this method to the detection of small amounts of S-methyldopa and R-carbidopa in S-carbidopa has been demonstrated.