ANTIBODIES TO CELL-SURFACE ANTIGENS OF PLANT-PROTOPLASTS

被引：10

作者：

LYNES, M ^{[1
]}

LAMB, CA ^{[1
]}

NAPOLITANO, LA ^{[1
]}

STOUT, RG ^{[1
]}

机构：

[1] MONTANA STATE UNIV, DEPT BIOL, BOZEMAN, MT 59717 USA

来源：

PLANT SCIENCE | 1987年 / 50卷 / 03期

关键词：

antibodies; Avena sativa; cell-surface antigens; Phaseolus vulgaris; plasma membrane; Zea mays;

D O I：

10.1016/0168-9452(87)90077-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Both mouse and rabbit polyclonal antibodies to plant cell-surface antigens were developed by immunization with cell membrane material from oat (Avena sativa L. cv. Garry) roots. We were able to quickly assess the activity of antisera by monitoring the degree of protoplast agglutination and by using an indirect immunofluorescence assay. Using polyclonal antibodies to cell-surface antigens, we have found that oat root protoplasts share common surface antigens with protoplasts from other plant tissues and species. From experiments with antisera treated with excess oat leaf or oat root protoplasts before our immunoassays, we have obtained evidence for the existence of organ-specific cell-surface antigens in higher plants. © 1987.

引用

页码：225 / 232

页数：8

共 13 条

[1]

Chua N. H., 1980, METHOD ENZYMOL, V69, P434, DOI 10.1016/S0076-6879(80)69042-9

[2] INDIA INK STAINING OF PROTEINS ON NITROCELLULOSE PAPER [J].