Advances in the Study of Heart Development and Disease Using Zebrafish

被引:99
作者
Brown, Daniel R. [1 ,3 ]
Samsa, Leigh Ann [2 ,3 ]
Qian, Li [1 ,3 ]
Liu, Jiandong [1 ,3 ]
机构
[1] Univ N Carolina, Dept Pathol & Lab Med, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Dept Cell Biol & Physiol, Chapel Hill, NC 27599 USA
[3] Univ N Carolina, McAllister Heart Inst, Chapel Hill, NC 27599 USA
基金
美国国家卫生研究院;
关键词
zebrafish; congenital heart disease; cardiomyopathy; cardiac arrhythmia; development; translational medicine; drug screens;
D O I
10.3390/jcdd3020013
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Animal models of cardiovascular disease are key players in the translational medicine pipeline used to define the conserved genetic and molecular basis of disease. Congenital heart diseases (CHDs) are the most common type of human birth defect and feature structural abnormalities that arise during cardiac development and maturation. The zebrafish, Danio rerio, is a valuable vertebrate model organism, offering advantages over traditional mammalian models. These advantages include the rapid, stereotyped and external development of transparent embryos produced in large numbers from inexpensively housed adults, vast capacity for genetic manipulation, and amenability to high-throughput screening. With the help of modern genetics and a sequenced genome, zebrafish have led to insights in cardiovascular diseases ranging from CHDs to arrhythmia and cardiomyopathy. Here, we discuss the utility of zebrafish as a model system and summarize zebrafish cardiac morphogenesis with emphasis on parallels to human heart diseases. Additionally, we discuss the specific tools and experimental platforms utilized in the zebrafish model including forward screens, functional characterization of candidate genes, and high throughput applications.
引用
收藏
页数:25
相关论文
共 194 条
[1]   Aquatic surface respiration and swimming behaviour in adult and developing zebrafish exposed to hypoxia [J].
Abdallah, Sara J. ;
Thomas, Benjamin S. ;
Jonz, Michael G. .
JOURNAL OF EXPERIMENTAL BIOLOGY, 2015, 218 (11) :1777-1786
[2]   A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish [J].
Ablain, Julien ;
Durand, Ellen M. ;
Yang, Song ;
Zhou, Yi ;
Zon, Leonard I. .
DEVELOPMENTAL CELL, 2015, 32 (06) :756-764
[3]   Ionic channels underlying the ventricular action potential in zebrafish embryo [J].
Alday, Aintzane ;
Alonso, Hiart ;
Gallego, Monica ;
Urrutia, Janire ;
Letamendia, Ainhoa ;
Callol, Caries ;
Casis, Oscar .
PHARMACOLOGICAL RESEARCH, 2014, 84 :26-31
[4]   The zebrafish as a tool to identify novel therapies for human cardiovascular disease [J].
Asnani, Aarti ;
Peterson, Randall T. .
DISEASE MODELS & MECHANISMS, 2014, 7 (07) :763-767
[5]  
Auer T.O., 2014, METHODS, P69
[6]   CRISPR/Cas9 and TALEN-mediated knock-in approaches in zebrafish [J].
Auer, Thomas O. ;
Del Bene, Filippo .
METHODS, 2014, 69 (02) :142-150
[7]   Direct and indirect roles for Nodal signaling in two axis conversions during asymmetric morphogenesis of the zebrafish heart [J].
Baker, Kari ;
Holtzman, Nathalia G. ;
Burdine, Rebecca D. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (37) :13924-13929
[8]   Individual variation in the rate of oxygen consumption by zebrafish embryos [J].
Bang, A ;
Gronkjær, P ;
Malte, H .
JOURNAL OF FISH BIOLOGY, 2004, 64 (05) :1285-1296
[9]   O2 consumption and heart rate in developing zebrafish (Danio rerio):: influence of temperature and ambient O2 [J].
Barrionuevo, WR ;
Burggren, WW .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1999, 276 (02) :R505-R513
[10]   Zebrafish: an emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery [J].
Barros, T. P. ;
Alderton, W. K. ;
Reynolds, H. M. ;
Roach, A. G. ;
Berghmans, S. .
BRITISH JOURNAL OF PHARMACOLOGY, 2008, 154 (07) :1400-1413