TISSUE-CULTURE STUDY ON MONGOLIAN GERBILS (MERIONES-UNGUICULATUS) MALIGNANT-MELANOMA

被引:4
作者
MORITOMO, T
SAITO, H
WATANABE, T
MOCHIZUKI, K
机构
[1] Laboratory of Fish Pathology, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa
关键词
CELL LINE; MELANOMA; MONGOLIAN GERBIL; TISSUE CULTURE;
D O I
10.1538/expanim1978.40.3_385
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
We tried to culture melanoma cells from a Mongolian gerbil's (Meriones unguiculatus) malignant tumor. In primary culture, most of cells have abundant melanin granules in their cytoplasm. Melanin granules decreased through 5 to 15 serial passages and disappeared after 15 th passage. The morphology of the cells varied from spindle to large polydendritic cells. Although typical melanin granules were not seen when the cells were stained by Masson-Fontana method, the cells were positive for DOPA reaction. Electron microscopically, most of the cells have well-developed Golgi apparatus and rough-surfaced endoplasmic reticulum with dilated cistern, and premelanosome-like granules were frequently observed in their cytoplasm.
引用
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页码:385 / 388
页数:4
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