CONSTRUCTION OF AN INTEGRATION VECTOR FOR USE IN THE ARCHAEBACTERIUM METHANOCOCCUS-VOLTAE AND EXPRESSION OF A EUBACTERIAL RESISTANCE GENE

被引：105
作者：
GERNHARDT, P
POSSOT, O
FOGLINO, M
SIBOLD, L
KLEIN, A
机构：
[1] UNIV MARBURG,FACHBEREICH BIOL,POSTFACH 1929,W-3550 MARBURG,GERMANY
[2] INST PASTEUR,DEPT BIOTECHNOL,CNRS,UNITE PHYSIOL CELLULAIRE & LIRA 1300,F-75724 PARIS 15,FRANCE
来源：
MOLECULAR & GENERAL GENETICS | 1990年 / 221卷 / 02期
关键词：
Expression of heterologous resistance gene; Integration vector; Methanococcus voltae; Transformation; Vector stability;
D O I：
10.1007/BF00261731
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An integration vector for use in Methanococcus voltae was constructed, based on the Escherichia coli vector pUC18. It carries the structural gene for puromycin transacetylase from Streptomyces alboniger, which is flanked by expression signals of M. voltae structural genes and hisA gene sequences of this bacterium. Transformed M. voltae cells are puromycin resistant. Several types of integration of the vector into the chromosome were found. Only one case was due to nonhomologous recombination. The integrated sequences were stable under selective pressure but were slowly lost in some cases in the absence of the selective drug. The vector could be excised from M. voltae chromosomal DNA, recircularized and transformed back into E. coli. © 1990 Springer-Verlag.
引用
收藏
页码：273 / 279
页数：7
相关论文
共 31 条
[21]   THE DOUBLE-STRAND-BREAK REPAIR MODEL FOR RECOMBINATION [J].
SZOSTAK, JW ;
ORRWEAVER, TL ;
ROTHSTEIN, RJ ;
STAHL, FW .
CELL, 1983, 33 (01) :25-35
[22]   HIGH-FREQUENCY TARGETING OF GENES TO SPECIFIC SITES IN THE MAMMALIAN GENOME [J].
THOMAS, KR ;
FOLGER, KR ;
CAPECCHI, MR .
CELL, 1986, 44 (03) :419-428
[23]   MVNI - A RESTRICTION ENZYME IN THE ARCHAEBACTERIUM METHANOCOCCUS-VANNIELII [J].
THOMM, M ;
FREY, G ;
BOLTON, BJ ;
LAUE, F ;
KESSLER, C ;
STETTER, KO .
FEMS MICROBIOLOGY LETTERS, 1988, 52 (03) :229-234
[24]   CLONING AND EXPRESSION OF A PUROMYCIN N-ACETYL TRANSFERASE GENE FROM STREPTOMYCES-ALBONIGER IN STREPTOMYCES-LIVIDANS AND ESCHERICHIA-COLI [J].
VARA, J ;
MALPARTIDA, F ;
HOPWOOD, DA ;
JIMENEZ, A .
GENE, 1985, 33 (02) :197-206
[25]  
VARA JA, 1986, EXPRESSION MAMMALIAN
[26]   NUTRITION AND CARBON METABOLISM OF METHANOCOCCUS-VOLTAE [J].
WHITMAN, WB ;
ANKWANDA, E ;
WOLFE, RS .
JOURNAL OF BACTERIOLOGY, 1982, 149 (03) :852-863
[27]   COMPLEMENTATION OF ARGG AND HISA MUTATIONS OF ESCHERICHIA-COLI BY DNA CLONED FROM THE ARCHAEBACTERIUM METHANOCOCCUS-VOLTAE [J].
WOOD, AG ;
REDBORG, AH ;
CUE, DR ;
WHITMAN, WB ;
KONISKY, J .
JOURNAL OF BACTERIOLOGY, 1983, 156 (01) :19-29
[28]   A NEWLY-ISOLATED MARINE METHANOGEN HARBORS A SMALL CRYPTIC PLASMID [J].
WOOD, AG ;
WHITMAN, WB ;
KONISKY, J .
ARCHIVES OF MICROBIOLOGY, 1985, 142 (03) :259-261
[29]   IMPROVED M13 PHAGE CLONING VECTORS AND HOST STRAINS - NUCLEOTIDE-SEQUENCES OF THE M13MP18 AND PUC19 VECTORS [J].
YANISCHPERRON, C ;
VIEIRA, J ;
MESSING, J .
GENE, 1985, 33 (01) :103-119
[30]  
YOUNG M, 1984, J GEN MICROBIOL, V130, P1613
1234