FLUORIMETRIC METHOD FOR CONTINUOUSLY ASSAYING ATPASE - APPLICATION TO SMALL SPECIMENS OF GLYCEROL-EXTRACTED MUSCLE-FIBERS

被引：55
作者：
TAKASHI, R
PUTNAM, S
机构：
[1] Cardiovascular Research Institute, University of California, San Francisco
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 02期
关键词：
D O I：
10.1016/0003-2697(79)90674-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A continuous fluorimetric method using auxiliary-coupling enzymes such as pyruvate kinase and lactate dehydrogenase for measuring ADP production to assay ATPase activity is described. This method is simpler, more rapid, and more sensitive than the previously used spectrophotometric method. The application of this method for studying the ATPase of rabbit psoas muscle fibers during Mg2+-ATP activation is also illustrated and discussed. © 1979.
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页码：375 / 382
页数：8
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