DETERGENT-LIKE EFFECTS OF ALAMETHICIN ON LYMPHOCYTE PLASMA-MEMBRANES

被引：26
作者：
BONNAFOUS, JC
DORNAND, J
MANI, JC
机构：
[1] Laboratoire de Biochimie, Membranes Ecole Nationale Supérieure de Chimie, F-34075 Montpellier, 8, rue de l'Ecole Normale
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 03期
关键词：
D O I：
10.1016/0006-291X(79)91747-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Alamethicin enhanced adenylate cyclase and Na+-K+-ATPase activities in microsomes and purified plasma membranes from pig lymphocytes. As this stimulation was also found in inside-out vesicles obtained from these membranes and as we showed that lymphocyte membrane vesicles are not impermeable to 5′AMP, ATP and concanavalin A, it appears clearly that alamethicin effects are not related to its channel-forming properties, but rather to its detergent-like character. Indeed sodium dodecylsulfate and Lubrol PX mimicked alamethicin effects. Moreover alamethicin treatment of plasma membranes induced protein and phospholipid solubilization. © 1979.
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页码：536 / 544
页数：9
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