KINETIC STUDIES ON FLAVOPROTEIN COMPONENT (E3) OF RAT-BRAIN PYRUVATE-DEHYDROGENASE MULTI-ENZYME COMPLEX

被引：1
作者：
NGO, TT
BARBEAU, A
机构：
[1] Clinical Research Institute of Montreal, Montreal, Que.
来源：
INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1978年 / 9卷 / 09期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1016/0020-711X(78)90095-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. 1. Kinetic studies were made with the effects of pyruvate and coenzyme A (CoA) on the reactions catalyzed by dihydrolipoate dehydrogenase (E3, a flavoprotein). 2. 2. Pyruvate does not have an appreciable effect on the reactions catalyzed by E3. However, CoA noncompetitively inhibits E3 with both lipoamide and NADH as the variable substrates. 3. 3. These results suggest the possible interaction between the component-enzymes of PDHC through their physical association and thus offer a possible explanation for the anomalous product inhibition experiments which were observed previously by us (Ngo & Barbeau, 1978). © 1978.
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页码：681 / 684
页数：4
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