STABILIZED BILAYER-LIPID MEMBRANES FOR FLOW-THROUGH EXPERIMENTS

This work reports a technique for the stabilization of solventless bilayer lipid membranes (BLMs), and the use of stabilized BLMs as flow detectors. Microporous filters composed of glass fibers, polytetrafluoroethylene (PTFE) and polycarbonate (nominal pore sizes from 1 to 5 mu m) can serve as interfaces that separate two solution compartments. The micropores in the filter media can act as supports for formation and stabilization of BLMs. One of the solution compartments is used to cast lipid films on the filters, while a carrier electrolyte solution concurrently hows through the opposing compartment. Optimization of the how cell design, and the chemical composition and methods for preparation of stabilized BLMs, are described. Lipid membranes composed of mixtures of phosphatidyl choline and phosphatidic acid could respond rapidly to pH alterations of the carrier electrolyte solution. Signals would reproducibly appear within a few seconds following the injection of an electrolyte of different pH than the carrier. Signals took the form of a single ion current transient with magnitude of tens of picoamperes (pA) and a duration of seconds. The mechanism of signal generation is explored by differential scanning calorimetry. The results show that a phase transition within a lipid membrane can be triggered by pH alterations of the electrolyte solution. Stabilized BLMs which provide artificial ion gating events hold prospects for chemical sensing of process streams.